Microtiter plate assay for sexual agglutination in the yeast Hansenula wingei

The spheroplast lysis assay for yeast in microtiter plate format.

A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for...

Inactivation of Sexual Agglutination in Hansenula Wingei and Saccharomyces Kluyveri by Disulfide-cleaving Agents.

Taylor, Neil W. (Northern Regional Research Laboratory, Peoria, Ill.). Inactivation of sexual agglutination in Hansenula wingei and Saccharomyces kluyveri by disulfide-cleaving agents. J. Bacteriol. 88:929-936. 1964.-Mating types of both Hansenula wingei and Saccharomyces kluyveri can be activated to produce uniformly strong sexual agglutination by treatments with various solvents, such as 8 m ...

Physiology of the conjugation process in the yeast Hansenula wingei.

The yeast Hansenula wingei is a favourable organism for study of the physiology of the conjugation process (cell fusion). Microscopic observations on fusion are presented which reveal that the mating cells in contact fuse by a softening of the cell wall, followed by formation of a conjugation tube, dissolution of the cross-walls between them, and formation of a new bud a t the point of juncture...

Microtiter plate agglutination test for Salmonella antibodies.

Similar results were obtained when testing human sera for Salmonella antibodies by the tube agglutination test and by the Microtiter plate agglutination test. The plate test was easier to perform and saved time, space, antigen, and serum.

Molecular basis of mating in the yeast hansenula wingei.